首页 / 院系成果 / 成果详情页

Overexpression of Glypican 3 Promotes Proliferation, Regulates Cell Cycle Progression, and Inhibits Apoptosis of Human Fetal Osteoblastic Cell Line 1.19 期刊论文

编号：

f43851da-5cec-410e-8616-819c99c2d96f
作者：

Cai, Tianyi(蔡天翼)#^[1]Wu, Yingzhi^[1];Ke, Ronghu(柯荣湖)^[1]Yang, Junyi(杨君毅)^[1]Ghanem, Abdulsamad^[1];Mu, Xiongzheng(穆雄铮)*^[1]
语种：

English
期刊：

JOURNAL OF CRANIOFACIAL SURGERY ISSN：1049-2275 2017 年 28 卷 6 期 (1481 - 1485) ; SEP
收录：
关键词：

GPC3 hFOBs osteoblast syndromic craniosynostosis
摘要：

Craniosynostosis is a complex disease condition, which involves premature fusion of cranial vault sutures and lacks desirable treatment. Previous studies have demonstrated decreased proliferation rate of osteoblasts and downregulated expression of glypican 3 (GPC3) in syndromic craniosynostosis patients. In this study, quantitative and qualitative analysis were utilized to assess the effect of GPC3 in human fetal osteoblastic cell line, hFOB 1.19. Lentiviral transfection efficiency with green fluorescent protein images was obtained after 72 hours. Western Blot and quantitative real-time polymerase chain reaction analysis results indicated that GPC3 was overexpressed in hFOB 1.19 cells transfected with recombinant lentivirus LV-GPC3-GFP. Cell proliferation was assessed by CCK-8 assay and cell cycle progression and apoptosis were analyzed by flow cytometric assay. Results revealed that GPC3 promoted cell viability, induced cell cycle entry into S phase, and inhibited cell apoptosis. These findings provide novel ideas in understanding the pathogenesis of craniosynostosis. It also provides novel insights in the treatment of craniosynostosis by targeting GPC3.
推荐引用方式
GB/T 7714：

Cai Tianyi,Wu Yingzhi,Ke Ronghu, et al. Overexpression of Glypican 3 Promotes Proliferation, Regulates Cell Cycle Progression, and Inhibits Apoptosis of Human Fetal Osteoblastic Cell Line 1.19 [J].JOURNAL OF CRANIOFACIAL SURGERY,2017,28(6):1481-1485.
APA：

Cai Tianyi,Wu Yingzhi,Ke Ronghu,Yang Junyi,&Mu Xiongzheng.(2017).Overexpression of Glypican 3 Promotes Proliferation, Regulates Cell Cycle Progression, and Inhibits Apoptosis of Human Fetal Osteoblastic Cell Line 1.19 .JOURNAL OF CRANIOFACIAL SURGERY,28(6):1481-1485.
MLA：

Cai Tianyi, et al. "Overexpression of Glypican 3 Promotes Proliferation, Regulates Cell Cycle Progression, and Inhibits Apoptosis of Human Fetal Osteoblastic Cell Line 1.19" .JOURNAL OF CRANIOFACIAL SURGERY 28,6(2017):1481-1485.

浏览次数：5  下载次数：0

分享到：

浏览次数：5

下载次数：0

打印次数：0