Cationic polymeric liposomes (CPLs) were successfully prepared using a tumor-targeting EGF protein modification for the systemic delivery of the p53 gene in ovarian cancer. These functional CPL nanoparticles (NPs) can be used as effective gene delivery vectors and are composed of an EGF derivative (EGF-GHDC), cholesterol, and DOPE, which have lower gene transfection efficiencies compared with Lipofectamine 2000. The increased therapeutic efficiency of p53 gene-loaded EGF-CPL was tested in SKOV3 cells and compared with p53 gene-loaded CPL and free p53 gene in solution. In addition, EGF-CPL (effective diameter: 95.01 +/- 4.05 nm; polydispersity index: 0.270) significantly enhanced luciferase gene expression in vivo 3 days post-injection of pGL-3/NPs complexes. In conclusion, EGF-targeted CPL has the potential for use as an effective drug delivery system.
[1]Fudan Univ, Dept Oncol, Minhang Branch, Shanghai Canc Ctr, Shanghai, Peoples R China;
[2]Shanghai Jiao Tong Univ, Suzhou Kowloon Hosp, Dept Oncol, Suzhou 215021, Peoples R China;
[3]Shanghai Jiao Tong Univ, Renji Hosp, Dept Oncol, Shanghai 200127, Peoples R China
(8.0+极速模式)