Glycoprotein (GP) is a major antigen of Crimean-Congo hemorrhagic fever virus (CCHFV), and binds to its receptor on the host cell and induces protective immunity in the host. The aim of this study is to identify all linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) of CCHFV using a modified overlapping peptide biosynthesis method. The eight fine BCEs (Gn-E1a, (543)RTQLV(547); E1b, (EIH555)-E-553; E1c, (IHEDSY559)-I-554; E1d, (557)DSYG(560); E2, (615)CKQGFC(620); E3a, (657)GDILVD(662); E3b, (662)DCSGGQQH(669), and E4, (678)LGCPNVPL(685)) were identified using the rabbit antisera, which all were recognized by serum from IgG-positive sheep CCHFV-infected naturally in Western blotting. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. These mapped epitopes of the CCHFV Gn would provide a basis for further the elucidation of CCHFV pathogenesis, and the development of CCHFV multi-epitope vaccines and detection reagents.
[1]Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, 14th Shengli Rd, Urumqi 830046, Peoples R China;
[2]Fudan Univ, Shanghai Inst Planned Parenthood Res, Key Lab Reprod Regulat NPFPC, Shanghai 200032, Peoples R China;
[3]Ctr Dis Control & Prevent Xinjiang Uygur Autonomo, Urumqi 830001, Peoples R China;
[4]Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China
(8.0+极速模式)