首页 / 院系成果 / 成果详情页

Critical role of histone demethylase Jumonji domain-containing protein 3 in the regulation of neointima formation following vascular injury 期刊论文

编号：

f92ee55a-2600-4144-916f-b884b549cc26
作者：

Luo, XiaoLing^[1];Yang, Di^[1];Wu, Weijun^[1];Long, Fen^[1];Xiao, ChenXi^[1];Qin, Ming^[1];Law, Betty YuenKwan^[2,3];Suguro, Rinkiko^[1];Xu, Xin(徐欣)^[4]Qu, LeFeng^[5];Liu, XinHua(刘新华)*^[1]Zhu, Yi Zhun(朱依谆)*^[1,2,3]
语种：

English
期刊：

CARDIOVASCULAR RESEARCH ISSN：0008-6363 2018 年 114 卷 14 期 (1894 - 1906) ; DEC 1
收录：
关键词：

Epigenetic regulation JMJD3 Vascular remodelling NADPH oxidase 4 Autophagy
摘要：

Aims Jumonji domain-containing protein 3 (JMJD3), also called lysine specific demethylase 6B (KDM6b), is an inducible histone demethylase which plays an important role in many biological processes, however, its function in vascular remodelling remains unknown. We aim to demonstrate that JMJD3 mediates vascular neointimal hyperplasia following carotid injury, and proliferation and migration in platelet-derived growth factor BB (PDGF-BB)-induced vascular smooth muscle cells (VSMCs). Methods and results By using both genetic and pharmacological approaches, our study provides the first evidence that JMJD3 controls PDGF-BB-induced VSMCs proliferation and migration. Furthermore, our in vivo mouse and rat intimal thickening models demonstrate that JMJD3 is a novel mediator of neointima formation based on its mediatory effects on VSMCs proliferation, migration, and phenotypic switching. We further show that JMJD3 ablation by small interfering RNA or inhibitor GSK J4 can suppress the expression of NADPH oxidase 4 (Nox4), which is correlated with H3K27me3 enrichment around the gene promoters. Besides, deficiency of JMJD3 and Nox4 prohibits autophagic activation, and subsequently attenuates neointima and vascular remodelling following carotid injury. Above all, the increased expression of JMJD3 and Nox4 is further confirmed in human atherosclerotic arteries plaque specimens. Conclusions JMJD3 is a novel factor involved in vascular remodelling. Deficiency of JMJD3 reduces neointima formation after vascular injury by a mechanism that inhibits Nox4-autophagy signalling activation, and suggesting JMJD3 may serve as a perspective target for the prevention and treatment of vascular diseases.
推荐引用方式
GB/T 7714：

Luo XiaoLing,Yang Di,Wu Weijun, et al. Critical role of histone demethylase Jumonji domain-containing protein 3 in the regulation of neointima formation following vascular injury [J].CARDIOVASCULAR RESEARCH,2018,114(14):1894-1906.
APA：

Luo XiaoLing,Yang Di,Wu Weijun,Long Fen,&Zhu Yi Zhun.(2018).Critical role of histone demethylase Jumonji domain-containing protein 3 in the regulation of neointima formation following vascular injury .CARDIOVASCULAR RESEARCH,114(14):1894-1906.
MLA：

Luo XiaoLing, et al. "Critical role of histone demethylase Jumonji domain-containing protein 3 in the regulation of neointima formation following vascular injury" .CARDIOVASCULAR RESEARCH 114,14(2018):1894-1906.

浏览次数：20  下载次数：0

分享到：

浏览次数：20

下载次数：0

打印次数：0