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Identification of gene products that control lipid droplet size in yeast using a high-throughput quantitative image analysis 期刊论文

编号：

fb59f193-ba54-4d7d-a684-a820ee5c1135
作者：

Lv, Xuchao^[1,2] Liu, Jiaming^[1,2] Qin, Yiran^[3,4] Liu, Yizhang^[1,2] Jin, Meijun^[1,2] Dai, Junbiao^[3,4] Chua, Boon Tin^[5] Yang, Hongyuan^[6] Li, Peng^[1,2]
语种：

英文
期刊：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS ISSN：1388-1981 2019 年 1864 卷 2 期 (113 - 127) ; FEB
收录：
关键词：

Lipid droplet High-content screen Quantitative image analysis Cidea FLD1/seipin Yeast
摘要：

Lipid droplets (LDs) are important organelles involved in energy storage and expenditure. LD dynamics has been investigated using genome-wide image screening methods in yeast and other model organisms. For most studies, genes were identified using two-dimensional images with LD enlargement as readout. Due to imaging limitation, reduction of LD size is seldom explored. Here, we aim to set up a screen that specifically utilizes reduced LD size as the readout. To achieve this, a novel yeast screen is set up to quantitatively and systematically identify genes that regulate LD size through a three-dimensional imaging-based approach. Cidea which promotes LD fusion and growth in mammalian cells was overexpressed in a yeast knockout library to induce large LD formation. Next, an automated, high-throughput image analysis method that monitors LD size was utilized. With this screen, we identified twelve genes that reduced LD size when deleted. The effects of eight of these genes on LD size were further validated in fid1 null strain background. In addition, six genes were previously identified as LD-regulating genes. To conclude, this methodology represents a promising strategy to screen for players in LD size control in both yeast and mammalian cells to aid in the investigation of LD-associated metabolic diseases.
推荐引用方式
GB/T 7714：

Lv Xuchao,Liu Jiaming,Qin Yiran, et al. Identification of gene products that control lipid droplet size in yeast using a high-throughput quantitative image analysis [J].BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,2019,1864(2):113-127.
APA：

Lv Xuchao,Liu Jiaming,Qin Yiran,Liu Yizhang,&Li Peng.(2019).Identification of gene products that control lipid droplet size in yeast using a high-throughput quantitative image analysis .BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,1864(2):113-127.
MLA：

Lv Xuchao, et al. "Identification of gene products that control lipid droplet size in yeast using a high-throughput quantitative image analysis" .BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1864,2(2019):113-127.

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