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Renal Protection Mediated by Hypoxia Inducible Factor-1 alpha Depends on Proangiogenesis Function of miR-21 by Targeting Thrombospondin 1 期刊论文

编号：

fb794e71-2f0e-472d-9ff7-4f9446a79d3f
作者：

Xu, Xialian(徐夏莲)#^[1,2,3]Song, Nana^[1,2,3];Zhang, Xue^[4];Jiao, Xiaoyan(焦晓燕)^[1,2,3]Hu, Jiachang(胡家昌)^[1,2,3]Liang, Mingyu^[5,6];Teng, Jie(滕杰)^[1,2,3]Ding, Xiaoqiang(丁小强)^[1,2,3]
语种：

English
期刊：

TRANSPLANTATION ISSN：0041-1337 2017 年 101 卷 8 期 (1811 - 1819) ; AUG
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摘要：

Background Angiogenesis contributes to the repair process after renal ischemia/reperfusion (I/R) injury. In the present study, we tested the role of miR-21 in the angiogenesis induced by hypoxia inducible factor (HIF)-1 through inhibiting a predicted target gene thrombospondin 1 (TSP-1). Methods To stabilize HIF-1, hypoxia (1% O-2 for 24 hours) was performed in human umbilical vein endothelial cells and cobalt chloride (CoCl2) was pretreated intraperitoneally 24 hours before renal I/R in mice. Locked nucleic acid modified anti-miR-21 and scrambled control was transfected with hypoxic cells or delivered into the mice via tail vein 1 hour before CoCl2 injection. The kidneys and blood were collected at 24 hours after reperfusion. Results HIF-1 induced by hypoxia and CoCl2 upregulated vascular endothelial growth factor and miR-21, and increased angiogenesis. It was found that expression of TSP-1 was inversely related with miR-21 in vitro and in vivo. Targeting of TSP-1 by miR-21 was further confirmed in vitro. Furthermore, HIF-1 improved renal function, accompanied with increased angiogenesis after I/R injury in mice. The protective effect of HIF-1 was attenuated by inhibition of miR-21. Conclusions HIF-1 induced angiogenesis by upregulating not only vascular endothelial growth factor but also miR-21 via inhibiting a novel target gene TSP-1. Both of them may contribute to the protective effect of HIF-1 on renal I/R injury.
推荐引用方式
GB/T 7714：

Xu Xialian,Song Nana,Zhang Xue, et al. Renal Protection Mediated by Hypoxia Inducible Factor-1 alpha Depends on Proangiogenesis Function of miR-21 by Targeting Thrombospondin 1 [J].TRANSPLANTATION,2017,101(8):1811-1819.
APA：

Xu Xialian,Song Nana,Zhang Xue,Jiao Xiaoyan,&Ding Xiaoqiang.(2017).Renal Protection Mediated by Hypoxia Inducible Factor-1 alpha Depends on Proangiogenesis Function of miR-21 by Targeting Thrombospondin 1 .TRANSPLANTATION,101(8):1811-1819.
MLA：

Xu Xialian, et al. "Renal Protection Mediated by Hypoxia Inducible Factor-1 alpha Depends on Proangiogenesis Function of miR-21 by Targeting Thrombospondin 1" .TRANSPLANTATION 101,8(2017):1811-1819.

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