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Enhanced Fibroblast Cellular Ligamentization Process to Polyethylene Terepthalate Artificial Ligament by Silk Fibroin Coating 期刊论文

编号：

fd1ae6aa-0d12-4302-b405-5c0b55d75ef3
作者：

Jiang, Jia^[1,2];Ai, Chengchong^[1];Zhan, Zufeng^[3];Zhang, Peng^[1];Wan, Fang^[1];Chen, Jun^[1];Hao, Wei^[4];Wang, Yaxian^[4];Yao, Jinrong^[4];Shao, Zhengzhong^[4];Chen, Tianwu(陈天午)^[1]Zhou, Liang^[5];Chen, Shiyi(陈世益)^[1]
语种：

English
期刊：

ARTIFICIAL ORGANS ISSN：0160-564X 2016 年 40 卷 4 期 (385 - 393) ; APR
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关键词：

Polyethylene terepthalate artificial ligament Silk fibroin Fibroblast cell Ligamentization
摘要：

Artificial ligaments utilized in reconstruction of anterior cruciate ligament (ACL) are usually made of polyethylene terepthalate (PET) because of its good mechanical properties in vivo. However, it was found that the deficiencies in hydrophilicity and biocompatibility of PET hindered the process of ligamentization. Therefore, surface modification of the PET is deemed as a solution in resolving such problem. Silk fibroin (SF), which is characterized by good biocompatibility and low immunogenicity in clinical applications, was utilized to prepare a coating on the PET ligament (PET+SF) in this work. At first, decrease of hydrophobicity and appearance of amino groups were found on the surface of artificial PET ligament after coating with SF. Second, mouse fibroblasts were cultured on the two different kinds of ligament in order to clarify the possible effect of SF coating. It was proved that mouse fibroblasts display better adhesion and proliferation on PET+SF than PET ligament according to the results of several technical methods including SEM observation, cell adhesive force and spread area test, and mRNA analysis. Meanwhile, methylthiazolyldiphenyl-tetrazolium bromide and DNA content tests showed that biocompatibility of PET+SF is better than PET ligament. In addition, collagen deposition tests also indicated that the quantity of collagen in PET+SF is higher than PET ligament. Based on these results, it can be concluded that SF coating is suggested to be an effective approach to modify the surface of PET ligament and enhance the ligamentization process in vivo accordingly.
推荐引用方式
GB/T 7714：

Jiang Jia,Ai Chengchong,Zhan Zufeng, et al. Enhanced Fibroblast Cellular Ligamentization Process to Polyethylene Terepthalate Artificial Ligament by Silk Fibroin Coating [J].ARTIFICIAL ORGANS,2016,40(4):385-393.
APA：

Jiang Jia,Ai Chengchong,Zhan Zufeng,Zhang Peng,&Chen Shiyi.(2016).Enhanced Fibroblast Cellular Ligamentization Process to Polyethylene Terepthalate Artificial Ligament by Silk Fibroin Coating .ARTIFICIAL ORGANS,40(4):385-393.
MLA：

Jiang Jia, et al. "Enhanced Fibroblast Cellular Ligamentization Process to Polyethylene Terepthalate Artificial Ligament by Silk Fibroin Coating" .ARTIFICIAL ORGANS 40,4(2016):385-393.

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