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Exendin-4, a glucagon-like peptide-1 receptor agonist, inhibits A beta(25-35)-induced apoptosis in PC12 cells by suppressing the expression of endoplasmic reticulum stress-related proteins 期刊论文

编号：

fd4bfe4a-f50e-47f6-b99e-52e79cae6248
作者：

Zhang, Jianfeng(张建峰)#^[1]Wu, Junfeng(邬军锋)^[1]Zeng, Weichen^[1];Zhao, Yongfei(赵永飞)^[1]Zu, Hengbing(祖衡兵)*^[1]
语种：

English
期刊：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY ISSN：1936-2625 2015 年 8 卷 10 期 (12784 - 12792)
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关键词：

Neurodegenerative disorders Exendin-4 endoplasmic reticulum stress A beta(25-35) apoptosis
摘要：

Neurodegenerative disorders are chronic and progressive disease. Exendin-4 (Ex-4) can function as a neuroprotective agent and has novel therapeutic ability for the treatment of neurodegenerative disorders. In this study, we aimed to explore the neuroprotective effect of Ex-4 on PC12 cell apoptosis induced by A beta(25-35) in molecular level. The apoptosis of PC12 cells was detected by MTT assay, TUNEL staining and flow cytometry. The expression of ERS (endoplasmic reticulum stress, ERS) related proteins such as CHOP, GRP78 and Caspase-12 were determined by Western blot and cell immunocytochemistry. Results showed the apoptotic rate of PC12 cells significantly increased after A beta(25-35). addition, which was remarkably reduced after Ex-4 treatment. The expression of CHOP, GRP78 and Caspase-12 were significantly upregulated, and then remarkably reduced after Ex-4 treatment, while in the presence of Exendin9-39, the effect of Ex-4 was reversed. In conclusion, endoplasmic reticulum stress might be involved in the apoptosis process of PC12 cell induced by A beta(25-35) 3 and Ex-4 might provide a potential strategy for the treatment and prevention of cell apoptosis-associated disorders.
推荐引用方式
GB/T 7714：

Zhang Jianfeng,Wu Junfeng,Zeng Weichen, et al. Exendin-4, a glucagon-like peptide-1 receptor agonist, inhibits A beta(25-35)-induced apoptosis in PC12 cells by suppressing the expression of endoplasmic reticulum stress-related proteins [J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2015,8(10):12784-12792.
APA：

Zhang Jianfeng,Wu Junfeng,Zeng Weichen,Zhao Yongfei,&Zu Hengbing.(2015).Exendin-4, a glucagon-like peptide-1 receptor agonist, inhibits A beta(25-35)-induced apoptosis in PC12 cells by suppressing the expression of endoplasmic reticulum stress-related proteins .INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,8(10):12784-12792.
MLA：

Zhang Jianfeng, et al. "Exendin-4, a glucagon-like peptide-1 receptor agonist, inhibits A beta(25-35)-induced apoptosis in PC12 cells by suppressing the expression of endoplasmic reticulum stress-related proteins" .INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 8,10(2015):12784-12792.

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