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Fully human single-domain antibody targeting a highly conserved cryptic epitope on the Nipah virus G protein 期刊论文

编号：

17603261FD71402F6C2AD7FE9E0E31D0
作者：

Wang, Yulu^[1,2];Sun, Yifang^[1,2];Shen, Zhaoling^[1,2];Wang, Cong^[1,2];Qian, Jun^[3]Mao, Qiyu^[1,2];Wang, Yajie^[1,2];Song, Wenping^[1,2];Kong, Yu^[1,2];Zhan, Changyou(占昌友)^[4,5,6]Chen, Zhenguo(陈振国)^[1,2]Dimitrov, Dimiter S.^[7]Yang, Zhenlin(杨振霖)^[8]Jiang, Shibo(姜世勃)^[1,2]Wu, Fan(吴凡)^[1,2]Lu, Lu(陆路)^[1,2]Ying, Tianlei(应天雷)^[1,2,9]Sun, Lei(孙磊)^[1,2,9]Wu, Yanling(吴艳玲)^[1,2,9]
语种：

英文
期刊：

NATURE COMMUNICATIONS ISSN：2041-1723 2024 年 15 卷 1 期 ; AUG 12
收录：
摘要：

Nipah virus infection, one of the top priority diseases recognized by the World Health Organization, underscores the urgent need to develop effective countermeasures against potential epidemics and pandemics. Here, we identify a fully human single-domain antibody that targets a highly conserved cryptic epitope situated at the dimeric interface of the Nipah virus G protein (receptor binding protein, RBP), as elucidated through structures by high-resolution cryo-electron microscopy (cryo-EM). This unique binding mode disrupts the tetramerization of the G protein, consequently obstructing the activation of the F protein and inhibiting viral membrane fusion. Furthermore, our investigations reveal that this compact antibody displays enhanced permeability across the blood-brain barrier (BBB) and demonstrates superior efficacy in eliminating pseudovirus within the brain in a murine model of Nipah virus infection, particularly compared to the well-characterized antibody m102.4 in an IgG1 format. Consequently, this single-domain antibody holds promise as a therapeutic candidate to prevent Nipah virus infections and has potential implications for vaccine development. The fully human single-domain antibody n425 aginst NiV targets a conserved cryptic epitope on G protein and inhibits membrane fusion by disrupting tetramerization. These insights advance neutralization mechanisms and effective countermeasure design.
推荐引用方式
GB/T 7714：

Wang Yulu,Sun Yifang,Shen Zhaoling, et al. Fully human single-domain antibody targeting a highly conserved cryptic epitope on the Nipah virus G protein [J].NATURE COMMUNICATIONS,2024,15(1).
APA：

Wang Yulu,Sun Yifang,Shen Zhaoling,Wang Cong,&Wu Yanling.(2024).Fully human single-domain antibody targeting a highly conserved cryptic epitope on the Nipah virus G protein .NATURE COMMUNICATIONS,15(1).
MLA：

Wang Yulu, et al. "Fully human single-domain antibody targeting a highly conserved cryptic epitope on the Nipah virus G protein" .NATURE COMMUNICATIONS 15,1(2024).
入库时间：

2025/2/19 14:06:24
更新时间：

2025/2/21 12:42:40

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