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Blocking the PD-1-PD-L1 axis by a novel PD-1 specific nanobody expressed in yeast as a potential therapeutic for immunotherapy 期刊论文

编号：

CA58FC4BA15AFA0F2ADE9626B3F3AB8C
作者：

Xian, Zongshu^[1];Ma, Linlin^[2];Zhu, Min^[3];Li, Guanghui^[3];Gai, Junwei^[3];Chang, Qing^[2];Huang, Yuliang^[1];Ju, Dianwen(鞠佃文)*^[1]Wan, Yakun^[3];
语种：

英文
期刊：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ISSN：0006-291X 2019 年 519 卷 2 期 (267 - 273) ; NOV 5
收录：
关键词：

PD-1 Nanobody P. pastoris Human serum albumin
摘要：

PD-1/PD-L1 pathway blocking with antibodies offers a vital and efficient therapeutic strategy to restore T cell-associated antitumor immunity and treats a variety of cancers in clinic. Nanobodies (Nbs) give several advantages over conventional monoclonal antibodies such as size, solubility, stability and costs. Additionally, P. pastoris is a suitable host for Nb production. Herein, we aim to produce and evaluate anti-PD-1 Nb derived from the P. pastoris. Our findings indicated that we successfully established the Nbs phage-displayed library against PD-1 with qualified library capacity and insert ratio. Anti-PD-1 Nb Nb97 was screened through PE-ELISA and flow cytometry. To extend half-life of Nb97, we contracted pPICZaA-Nb97-Nb97-HSA recombination vector, which was then transformed into the system of P. pastoris X-33. The yield of purified Nb97-Nb97-Human serum albumin (HSA) fused protein (MY2935) reached to 2.3 g/L after 147 h of fermentation. Meanwhile, the blocking effect of MY2935 is similar to that of MY2626 (humanized Nb97-Fc), and MY2935 showed better performance on stimulating the immune function through PD-1 reporter assay. Hence, P. pastoris X-33 expressing and secreting functional anti-PD-1 Nb-HSA fusion protein might be a system of high yield and low cost. (C) 2019 Published by Elsevier Inc.
推荐引用方式
GB/T 7714：

Xian Zongshu,Ma Linlin,Zhu Min, et al. Blocking the PD-1-PD-L1 axis by a novel PD-1 specific nanobody expressed in yeast as a potential therapeutic for immunotherapy [J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,519(2):267-273.
APA：

Xian Zongshu,Ma Linlin,Zhu Min,Li Guanghui,&Wan Yakun.(2019).Blocking the PD-1-PD-L1 axis by a novel PD-1 specific nanobody expressed in yeast as a potential therapeutic for immunotherapy .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,519(2):267-273.
MLA：

Xian Zongshu, et al. "Blocking the PD-1-PD-L1 axis by a novel PD-1 specific nanobody expressed in yeast as a potential therapeutic for immunotherapy" .BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 519,2(2019):267-273.
入库时间：

2020/4/12 0:29:36
更新时间：

2020/4/12 0:29:36

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