[1]Shanghai University of Traditional Chinese Medicine, Institute of Chinese Materia Medica,Shanghai,China
[2]Shanghai University of Traditional Chinese Medicine, Department of Chemistry,Shanghai,China
[3]Shanghai University of Traditional Chinese Medicine,Shanghai,China
[4]Shanghai University, Central Laboratory,Shanghai,China
[5]Fudan University, Central Laboratory,Shanghai,China
[6]University of Westminster, Department of Life Science,London,United Kingdom
[7]Shanghai University of Traditional Chinese Medicine, Department of Pharmacology,Shanghai,China
A simple and sensitive method for simultaneous determination of seven active constituents, jatrorrhizine, berberine, coptisine, palmatine, evodiamine, rutacarpine and paeoniflorin, from a Chinese medicine Wuji Pill in rat plasma was developed based on a liquid chromatography and tandem mass spectrometry method. The separation of these seven compounds was carried out on a Shiseido CAPCELL PAK C 18 column using a mobile phase consisting of acetonitrile (containing 0.1% formic acid and water (containing 0.1% formic acid and 10mmol/L ammonium acetate) and carbamazepine as an internal standard. Electrospray ionization in positive-ion mode and multiple reaction monitoring was used to identify and quantitate active components. All calibration curves gave good linearity (r>0.993) over the concentration range from 0.42-208.0ng/mL to 4.18-418.0ng/mL for all components. The precision of the in vivo study was evaluated by intra- and inter-day assays and the percentages of relative standard deviation were all within 15%. The method was successfully applied to pharmacokinetic study of all six alkaloids and one monoterpene in rat plasma after oral administration of the Wuji Pill. ? 2012 Elsevier B.V.
(8.0+极速模式)